Sven Mostböck

Hi, I just tried it on my computer with the latest version 0.9.22 and it works for me. Please see the attached image, which shows the dialog as it looks for me. So right now I can't say what the problem might be. Can you please describe it in more detail, and possible add a screenshot and example data? Thanks, Sven

Hi, I just tried it on my computer with the latest version 0.9.22 and it works for me. Please see the attached image, which shows the dialog as it looks for me. So right now I can't say what the problem might be. Can you please describe it in more detail, and possible add a screenshot and example data? Thanks, Svev

FCX files are FCSalyzers internal documents for analysis. Into these documents, plots are based that display the data of selected FCS files. Please read the Wiki and watch the tutorial videos: https://youtu.be/h8LTHK26lHM

Hi Scott, unfortunately that is not currently possible (neither of the two options you suggested). I can put in on my ToDo, but as you can see from the releases, I somehow never get around to actually implement changes. Sorry and I hope that you can still get some use out of FCSalyzer. Best regards, Sven

Dear Giovanna, I don't quite understand the question. What do you mean with " some ive results in mean or median calculation"? Thanks, Sven

Hi Ethan, thanks, this is another example that tells me I should do something about the axis limits. Your plot is interesting, as it seems that that FCS file mentions a range of 0-10000, but actually records the data only up to a little more than 4000 (probably 4096). Say, could you maybe share an example file for me for taking a look at it? Thanks, Sven

Hi Dominic, it is nice to hear that FCSalyzer is of use to you! I just gave it a try and I can see what you mean. Here a few comments: 1) Yes, I see that this is annoying when analysing a lot of different files. Yes, your suggestions are reasonable and should not be too difficult to implement. However, as you can see from the releases, development has slowed down horribly in the last few years. The reason is mainly that other activities have taken up a lot of my private time. I really must kick myself...

Hi Simona, I see a few options: 1) recommended solution: make a separate analysis for each experiment. If I understand you right, these were different data sets from separate experiments, probably performed on different days, maybe even on different FACS machines. I think the best way is to simply analyse them separately (using the same gating strategy). FACS data will differ from experiment to experiment, simply because of variability due to variations in performing the experiment and acquiring...