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Requirements
SOAPindel2 needs two input data sources:
The reference sequence file used to align the reads. It must be in Fasta format.
The files with read-alignments. SOAPindel2 accepts only Indexed BAM formats as input. Users can use the tools/soap2sam.cc to convert SOAP format to SAM format and use samtools convert SAM to indexed BAM. SOAPindel2 can guess the library insert sizes by itself.
Sequencing platform:Theoretically, SOAPindel2 is designed for all paired-end sequencing data because it doesn’t consider any qualities for now. So it works for both Ilumina GA data and 454 data, but we’ve only tested it on Ilumina GA data.
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