123FASTQ performs all the pre-processes of Illumina next-generation sequencing reads (FASTQ files) easier than ever.
Download the quick user manual for the latest version:
https://dl.adbioinformatics.net/NGSNeeds/myTools/123Fastq_v1.3_Manual.pdf
Authors:
Milad Eidi, Samaneh Abdolalizadeh, Mohammad Hossein Nassirpour
Supervisors:
Javad Zahiri, PhD
University of California San Diego
Masoud Garshasbi, PhD
Tarbiat Modares University, Tehran, Iran
If you use 123FASTQ, please cite this preprint:
123FASTQ: an intuitive and efficient tool for preprocessing Illumina FASTQ reads
https://www.biorxiv.org/content/10.1101/2024.03.08.584032v1
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Take care of the details and ensure you use the latest version.
We hope this helps...
Features
- Next generation sequencing reads quality check
- Quality check of paired-end reads
- Flexible trimmer
- Trim recommendations based on quality check results
- Adapter decontamination by two approaches
- Fast5 to FASTQ conversion
- SAM/BAM to FASTQ conversion
- Cross platform (unix, windows)
- Written in java