Primer3 - PCR primer design tool download

Design PCR primers from DNA sequence. Widely used (190k Google hits for "primer3"). From mispriming libraries to sequence quality data to the generation of internal oligos, primer3 does it. C&perl. Developers/testers/documenters needed.

Project Samples

Project Activity

See All Activity >

License

GNU General Public License version 2.0 (GPLv2)

Follow Primer3 - PCR primer design tool

Primer3 - PCR primer design tool Web Site

Other Useful Business Software

Gen AI apps are built with MongoDB Atlas

Build gen AI apps with an all-in-one modern database: MongoDB Atlas

MongoDB Atlas provides built-in vector search and a flexible document model so developers can build, scale, and run gen AI apps without stitching together multiple databases. From LLM integration to semantic search, Atlas simplifies your AI architecture—and it’s free to get started.

Start Free

Rate This Project

User Ratings

5.0 out of 5 stars

★★★★★

★★★★

★★★

★★

★

ease 1 of 5 2 of 5 3 of 5 4 of 5 5 of 5 0 / 5

features 1 of 5 2 of 5 3 of 5 4 of 5 5 of 5 0 / 5

design 1 of 5 2 of 5 3 of 5 4 of 5 5 of 5 0 / 5

support 1 of 5 2 of 5 3 of 5 4 of 5 5 of 5 0 / 5

User Reviews

Filter Reviews:

All

lisakane Posted 2013-06-10

Primer3 works perfectly.

1 user found this review helpful.
ryanhouse Posted 2013-06-09

Primer3 is perfect! Thanks.

1 user found this review helpful.
maria1985 Posted 2012-09-12

I use this every single day! I really liked it.
permeadycu Posted 2012-06-08

must have app for people on the go.
rieclothakspid Posted 2012-06-06

It is an awesome small and easy to use program

Additional Project Details

Operating Systems

Linux, BSD, Windows

Intended Audience

Science/Research

User Interface

Web-based, Command-line

Programming Language

Perl, C

Related Categories

Perl Bio-Informatics Software, C Bio-Informatics Software

Registered

2004-06-20

Similar Business Software

Geneious

Geneious Prime makes bioinformatics accessible by transforming raw data into visualizations that make sequence analysis intuitive and user-friendly. Simple sequence assembly and easy editing of contigs. Automatic annotation for gene prediction, motifs, translation, and variant calling. Genotype...

See Software
Genome Analysis Toolkit (GATK)

Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools with a primary focus on variant discovery and genotyping. Its powerful processing engine and high-performance computing features make it capable of taking on projects of any size. The GATK...

See Software
MEGA

MEGA (Molecular Evolutionary Genetics Analysis) is a powerful and user-friendly software suite designed for analyzing DNA and protein sequence data from species and populations. It facilitates both automatic and manual sequence alignment, phylogenetic tree inference, and evolutionary hypothesis...

See Software