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The Metawatt binner is a graphical binning tool that makes use of multivariate statistics of tetranucleotide frequencies and differential coverage based binning. It also performs taxonomic assessment of binning quality (via diamond BLASTx). Created bins can be edited and exported as fasta. The Metawatt is implemented in Java SWING and minimally depends on Diamond, HMMer3.1, BBMap, Prodigal and the Batik library for the export of SVG graphics.
Citation: Strous M, Kraft B, Bisdorf R, TegetMeyer H (2012) The binning of metagenomic contigs for microbial physiology of mixed cultures. Frontiers in Microbial Physiology and Metabolism 3:410. doi: 10.3389/fmicb.2012.00410
Features
- Combined tetranucleotide and differential coverage binning of metagenomic contigs
- Graphical user interface for data exploration
- Taxonomic Profiling of Bins with Diamond blastp
- Detection of Conserved Single Copy Genes
- Phylogenetic Treeing of bins based on conserved genes
- Interactive GC/coverage or coverage/coverage plots
- Also runs fully automatically on the command line
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Categories
Bio-InformaticsLicense
Academic Free License (AFL)Follow metawatt
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User Reviews
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I really like the program. It was easy to install and to run. it will be good if they can support bigger samples (it runs out of memory with my 1.2 gb files) or have a batch mode to work with all samples from a folder. Overall a useful program