easy-dhpsf

This package of MATLAB routines with accompanying GUI allows scientific users to measure the 3D location of single molecules when using the double-helix point spread function (DH-PSF) widefield microscope. Version 2 also includes code that facilitates combining localization data from two spectral channels using a locally-weighted quadratic 3D registration function

Features
- Double-Gaussian estimator is calibrated via an axial scan of bright immobile fluorescent emitters (e.g. beads)
- DH localization is performed through template matching and subsequent double Gaussian fitting
- Tiff stacks of SM images are analyzed using template matching followed by double-Gaussian fitting to extract estimates of the molecule positions
- Two spectral channels are registered by generating a locally-weighted quadratic mapping function from control point pairs

Project Activity

See All Activity >

License

BSD License

Follow easy-dhpsf

easy-dhpsf Web Site

Other Useful Business Software

MongoDB Atlas runs apps anywhere

Deploy in 115+ regions with the modern database for every enterprise.

MongoDB Atlas gives you the freedom to build and run modern applications anywhere—across AWS, Azure, and Google Cloud. With global availability in over 115 regions, Atlas lets you deploy close to your users, meet compliance needs, and scale with confidence across any geography.

Start Free

Rate This Project

User Reviews

Be the first to post a review of easy-dhpsf!

Additional Project Details

Intended Audience

Science/Research

Programming Language

MATLAB

Related Categories

MATLAB Molecular Science Software, MATLAB Chemistry Software, MATLAB Physics Software

Registered

2015-09-05

Similar Business Software

ActCAD Software

ActCAD is a native dwg/dxf cad software suitable for professional 2D drafting and 3D modeling projects. ActCAD is trusted by over 30000 users in over 103 countries for more than 10 years. The interface, commands, icons, dialogs, shortcuts etc. are very much similar to other popular cad software...

See Software
Houzz Pro

Houzz Pro is the #1 construction management solution for residential contractors and designers. Get an all-in-one solution that spans the full customer lifecycle, including marketing, CRM, estimates, takeoffs, 3D floor plans, project management, selections, online invoicing & payments,...

See Software
Pylon

Create accurate proposals from anywhere in under 2 minutes using hi-res imagery, energy analysis and 3D shading tools in Pylon's intuitive design studio. Pylon is the only solar design software to give you high-resolution imagery in-app with no monthly fees. Identify dates of concern and track...

See Software
SDS Manager

SDS Manager is a leading provider of Safety Data Sheet (SDS) management systems, offering one of the largest SDS databases with over 14 million documents available in 25 languages. With SDS Manager, employees can easily access Safety Data Sheets directly from their mobile devices by scanning...

See Software
AvPro Software

AvPro Software is comprehensive and easy-to-use. It's perfect for Aircraft MRO, Certified Repair Station (CRS), Aircraft Operators, and parts brokers. You can track Aircraft Parts I(nventory, Work Orders, and much more. Modular in nature and specifically designed for aircraft maintenance...

See Software
Dronedesk

Are you wasting hours on drone flight planning? Still using spreadsheets, doc templates, and paper checklists? If so, it's time to switch to Dronedesk, the web-based drone operations management application that makes planning safe drone flights super-efficient. Dronedesk does all the...

See Software