As per the title but as I have never worked with .wiff files before or the instruments that create them I need a little bit of help figuring out the issue here even though I think it is human (more specifically: people sending me a garbage .wiff file.)
MSconvert is producing the "errors" (again probably not errors, but correct analysis) in the attached picture.
From what I can tell, basically the issue is this is a .wiff file, but it is not one that actually has any chromatograms in it, just a bunch of other data possibly related to a run or some runs packed up into a .wiff format. Thus there is nothing for MSconvert to convert because there isn't any LC/MS data available in this .wiff file even though it is a pefectly valid .wiff file that could be used to contain LC/MS data, but isn't used that way by its creator.
I tried one other .wiff conversion tool and got a similar result, it produced no errors but it flopped out a .mzML file that was completely empty. This again implies to me this is a valid .wiff file, but someone sent me a .wiff file that while it may contain a whole bunch of experimental data (its over 6 MB), it does not contain a single bit of LC/MS data. I.e. the real problem is I "have been had" on the human side of things and was sent a non-LC/MS-data-containg .wiff file even though the people who sent it to me are highly insistent that this contains the chromatograms I need to analyze for them.
Nope the file is fine, the person who supplied it to me showed me via a bunch of screen shots that there are indeed a bunch of perfectly good chromatograms in it via an officiall ABI/Sciex program. Is the problem that MS convert is having is that there are multiple chromatograms all compacted into the same .wiff file? Is there any way to get MSconvert to handle this? Do I need to find some way to break it into lots of little .wiff files before MSconvert will handle it?
Any thoughts or help would be much appreciated in try to get at this .wiff file.
Thanks.
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Hi Nathaniel,
I work with a lot of .wiff files... Do you also have the wiff.scan files? There must be a .wiff and a .wiff.scan file with the same name in the directory for MSConvert to convert the files. Hope this helps.
If you would like to refer to this comment somewhere else in this project, copy and paste the following link:
As per the title but as I have never worked with .wiff files before or the instruments that create them I need a little bit of help figuring out the issue here even though I think it is human (more specifically: people sending me a garbage .wiff file.)
MSconvert is producing the "errors" (again probably not errors, but correct analysis) in the attached picture.
From what I can tell, basically the issue is this is a .wiff file, but it is not one that actually has any chromatograms in it, just a bunch of other data possibly related to a run or some runs packed up into a .wiff format. Thus there is nothing for MSconvert to convert because there isn't any LC/MS data available in this .wiff file even though it is a pefectly valid .wiff file that could be used to contain LC/MS data, but isn't used that way by its creator.
I tried one other .wiff conversion tool and got a similar result, it produced no errors but it flopped out a .mzML file that was completely empty. This again implies to me this is a valid .wiff file, but someone sent me a .wiff file that while it may contain a whole bunch of experimental data (its over 6 MB), it does not contain a single bit of LC/MS data. I.e. the real problem is I "have been had" on the human side of things and was sent a non-LC/MS-data-containg .wiff file even though the people who sent it to me are highly insistent that this contains the chromatograms I need to analyze for them.
Any thoughts or help would be appreciated.
Thanks.
UPDATE:
Nope the file is fine, the person who supplied it to me showed me via a bunch of screen shots that there are indeed a bunch of perfectly good chromatograms in it via an officiall ABI/Sciex program. Is the problem that MS convert is having is that there are multiple chromatograms all compacted into the same .wiff file? Is there any way to get MSconvert to handle this? Do I need to find some way to break it into lots of little .wiff files before MSconvert will handle it?
Any thoughts or help would be much appreciated in try to get at this .wiff file.
Thanks.
Hi Nathaniel,
I work with a lot of .wiff files... Do you also have the wiff.scan files? There must be a .wiff and a .wiff.scan file with the same name in the directory for MSConvert to convert the files. Hope this helps.